ASSESMENT OF ANTI-NOCICEPTIVE ACTIONS OF ADIPOSE-DERIVED MESEMCHYMAL STEM CELLS IN EXPERIMENTAL PERIPHERAL NEUROPATHIC PAIN

Objective. To estimate an anti-nociceptive and regenerative potential of adipose-derived mesenchymal stem cells in experimental post-traumatic neuropathy rats. Methods. Neuropathic pain was induced by axotomy technique rat left hind paw (Wistar rats (n=113)). The respective group subjects received ADMSCs dose 1×10<sup>6</sup> cells/kg 2×10<sup>6</sup> into the site sciatic nerve injury at 2 regimens: single (7<sup>th</sup> day post-surgery) twice 14<sup>th</sup> post-surgery). Nociceptive responses, as well histological changes perineural tissue were assessed dynamics. Results. Sciatic led to a significant increase mechanical nociceptive sensitivity ipsilateral 7<sup>th</sup> day, fibrotic peri- epineural areas damaged fibers denervation surrounding muscle fascia. Local administration effectively abolished hyperalgesia after first injection all regimens tested. Among tested regimens, most pronounced effects (1×10<sup>6</sup> cells/kg). As frequency elevated, their reparative anti-inflammatory properties reduced. Conclusion. Obtained results testify feasibility its further investigation on models neuropathy. What this paper adds For time impact different regimen allogenic (ADMSCs) transplantation microstructure with peripheral has been studied. Allogenic found out exhibit powerful local confirmed algometry study.